Phenotypic and genotypic pyrethroidresistance ofAedes albopictus, with focus onthe 2017 chikungunya outbreak in Italy

CAA Centro Agricoltura Ambiente > Scientific Papers > Sanitary entomology and zoology > Phenotypic and genotypic pyrethroidresistance ofAedes albopictus, with focus onthe 2017 chikungunya outbreak in Italy

Pichler V, C Malandruccolo ,P Serini, R Bellini, F Severini, L Toma, M Di Luca, F Montarsi, M Ballardini, M Manica, V Petrarca, J Vontas, S Kasai, A della Torrea, B Caputo

Pest Manag (2019)

BACKGROUND: The highly invasive mosquito species Aedes albopictus has become a major health concern in temperate areasdue to its role as vector of exotic arboviruses. Pyrethroid insecticides represent the main tools for limiting the circulation of such mosquito-borne viruses. The present work aim to extend previous reports on phenotypic pyrethroid-resistance in European Ae.albopictus, to identify its genetic basis and to monitor the geographical distribution of resistant genotypes, with a particularfocus on sites experiencing the 2017 chikungunya outbreak in Italy.
RESULTS: Bioassays, performed according to World Health Organization protocols, showed full susceptibility to deltamethrin (concentration=0.05%) and varying levels of resistance to permethrin (0.75%) and/or alfa-cypermethrin (0.05%) across Italy,with highest levels in the core of the 2017 chikungunya outbreak. Partial genotyping of the VSSC gene revealed widespread distribution of  V1016G mutation and confirmed its association with pyrethroid resistance.
CONCLUSION: The results obtained show that the condition for the spread of pyrethroid resistance in Ae. albopictus in Europe exists under strong selective pressure due to intensive insecticide spraying to control exotic arbovirus outbreak or high levelsof nuisance. The results draw attention to the need for an evidence-based implementation of mosquito nuisance control, taking insecticide resistance management into consideration.

 

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